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dhbF [2019-04-16 12:27:40]
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dhbF [2019-04-16 12:27:40]

involved in 2,3-dihydroxybenzoate biosynthesis
Locus
BSU31960
Isoelectric point
4.74
Molecular weight
263.44 kDa
Protein length
2378 aa Sequence Blast
Gene length
7137 bp Sequence Blast
Function
biosynthesis of the siderophore bacillibactin
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Homeostasis → Acquisition of iron → Acquisition of iron / Other
  • Metabolism → Additional metabolic pathways → Iron metabolism → Acquisition of iron / Other
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    3,280,519 3,287,655

    The protein

    Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Modification

  • phosphorylation on Ser-988 AND Ser-996 PubMed

    • Structure

  • 2VSQ (termination module of SrfAC, 31% identity) PubMed

    • Localization

  • membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigI: sigma factor, PubMed, in SigI regulon

    • Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • Kre: repression, in Kre regulon

    • Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

    • Additional information

  • the dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre mutant PubMed
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    Biological materials

    Mutant

  • MGNA-A063 (yukL::erm), available at the NBRP B. subtilis, Japan
  • BKE31960 (dhbF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC, downstream forward: _UP4_AATAAATAAGGGAGGAGAAA
  • BKK31960 (dhbF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC, downstream forward: _UP4_AATAAATAAGGGAGGAGAAA

    • References

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